Main description:

Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field.

Contents:

1. Intravital Multiphoton Microscopy for Dissecting Cellular Dynamics in Arthritic Inflammation and Bone Destruction

Junichi Kikuta and Masaru Ishii

2. Monitoring Multifunctionality of Immune-Exhausted CD8 T in Cancer Patients

Shingo Eikawa, Shusaku Mizukami, and Heiichiro Udono

3. Characterization of Innate Immune Signalings Stimulated by Ligands for Pattern Recognition Receptors

Takeshi Kameyama and Akinori Takaoka

4. Principled for the Use of in vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice

Norio Sakai

5. Unraveling Autoimmunity with the Adoptive Transfer of T Cells from TCR-Transgenic Mice

Huard Bertrand

6. In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets

Yumi Miyazaki and Shunichi Shiozawa

7. Characterization of microRNAs and Their Targets

Ghada Alsaleh and Jacques-Eric Gottenberg

8. Studies on the T Cell Receptor (TCR) Revision of Autoantibody-Inducing CD4T (aiCD4 T) Cell

Shunichi Shiozawa and Kenichi Uto

9. Basic Techniques for Studies of iNKT Cells and MAIT Cells

Asako Chiba and Sachiko Miyake

10. Induction of de novo Autoimmune Disease in Normal Mice Upon Repeated Immunization with Antigen

Ken Tsumiyama and Shunichi Shiozawa

11. Mouse Models of Experimental Dermal Fibrosis: The Bleomycin-Induced Dermal Fibrosis

Jerome Avouac

12. Screening for Novel Serum Biomarker for Monitoring Disease Activity in Rheumatoid Arthritis Using iTRAQ Technology Based Quantitative Proteomic Approach

Satoshi Serada and Tetsuji Naka

13. Genome-Wide Genetic Study in Autoimmune Disease Prone Mice

Masaomi Obata, Mareki Ohtsuji, Y. Iida, Toshikazu Shirai, Sachiko Hirose, and Hiroyuki Nishimura

14. Bayesian Systems-Based Genetic Association Analysis with Effect Strength Estimation and Omic Wide Interpretation: A Case Study in Rheumatoid Arthritis

Gabor Hullam, Andras Gezsi, Andras Millinghoffer, Peter Sarkoezy, Bence Bolgar, Sanjeev K. Srivastava, Zsuzsanna Pal, Edit I. Buzas, and Peter Antal

15. Sample Processing, Protocol and Statistical Analysis of the Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) of Protein, Cell and Tissue Samples

Goncalo Barreto, Antti Soininen, Tarvo Sillat, Yrjoe T. Kontinnen, and Emilia Kaivosoja

16. Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy

Antti Isomaki, Tarvo Sillat, Mari Ainola, Mikko Liljestroem, Yrjoe T. Konttinen, and Mika Hukkanen