Published December, 2010
By Greg Davis and Kevin J. Kayser
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
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Availability: Available

In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms.

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Published December, 2010
By Anton Posch
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
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Availability: Available

Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. The methods covered in this book are not limited to 2-DE;

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Published December, 2010
By Vladimir V. Didenko
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
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Availability: Available

Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others.

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Published December, 2010
By Tim D. Hewitson and Ian A. Darby
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
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Availability: Available

Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use.

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Published December, 2010
By Tarun B. Patel and Paul J. Bertics
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
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Availability: Available

A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states.

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Published December, 2010
By Minou Bina
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
Rating:
Availability: Available

More specifically, sequencing projects routinely deposit their data in p- licly available databases including GenBank, at the National Center of Biotechnology (NCBI) in the United States; Currently, several browsers offer facile access to numerous genomic DNA sequences for gene mapping and data retrieval.

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Published December, 2010
By Fernando Vivanco
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
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Availability: Available

Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.

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Published December, 2010
By John R. Stephenson and Alan Warnes
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
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Availability: Available

Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease.

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Published December, 2010
By Thomas Christopher Evans, Ming-Qun Xu and Thomas C. Evans, Jr.
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
Rating:
Availability: Available

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host.

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Published December, 2010
By Scott E. McNeil
Publisher: Springer (Humana Press Inc.)
Series: Methods in Molecular Biology
Rating:
Availability: Available

In Characterization of Nanoparticles Intended for Drug Delivery, expert researchers explore the latest advances in the field, providing a set of basic methods for the characterization of nanomaterials for medical use.

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