Main description:

This book provides a comprehensive introduction to all aspects of enzyme engineering, from fundamental principles through to the state-of-the-art in research and industrial applications.

It begins with a brief history, describing the milestones of advancement in enzyme science and technology, before going on to cover the fundamentals of enzyme chemistry, the biosynthesis of enzymes and their production. Enzyme stability and the reaction kinetics during enzymatic reactions are presented to show how enzymes function during catalysis and the factors that affect their activity. Methods to improve enzyme performance are also presented, such as cofactor regeneration and enzyme immobilization. The book emphasizes and elaborates on the performance and characteristics of enzymes at the molecular level. Finally, the book presents recent advances in enzyme engineering and some key industrial application of enzymes addressing the present needs of society.

This book presents essential information not only for undergraduate and graduate students, but also for researchers in academia and industry, providing a valuable reference for the development of commercial applications of enzyme technology.

Contents:

I. Introduction to Enzyme Engineering.

1. Introduction. 1.1 Brief History of Enzyme Engineering. 1.2 Industrial Application of Enzymes.

2. Biosynthesis of Enzymes. 2.1 Basic Enzyme Chemistry. 2.2 Biosynthesis of Enzymes.

3. Production of Enzymes. 3.1 Sources and Screening of Enzymes. 3.2 Production of Enzymes.

II. Enzyme Reaction Engineering.

4. Enzyme Reaction Kinetics. 4.1 Michaelis-Menten Kinetics. 4.2 Other Enzyme Kinetics.

5. Regeneration of Cofactors. 5.1 Regeneration of Reduced Nicotinamide Cofactors. 5.2 Regeneration of Oxidized Nicotinamide Cofactors.

6. Immobilized Enzymes. 6.1 Methods of Enzyme Immobilization. 6.2 Reactor System and Engineering Considerations.

7. Enzymes in Non-conventional Media. 7.1 Enzymes in Non-conventional Media. 7.2 Stability in Organic Solvent.

III. Molecular Understanding of Enzymes.

8. Engineering Tools for Enzymes. 8.1 Random Mutagenesis. 8.2 Rational and Computational Design.

9. Enzyme Catalysis. 9.1 Mechanism of Enzyme Activity. 9.2 Enzyme Dynamics and Flexibility.

10. Specificity of Enzymes. 10.1 Substrate Specificity. 10.2 Enantioselectivity of Enzymes.

11. Thermodynamics and Stability. 11.1 Enzyme Stability. 11.2 Stabilization of Enzymes.

IV. Application of Enzymes.

12. Enzymes for Chemicals and Polymers. 12.1 Enzymes for Chemicals. 12.2 Enzymes for Polymers.

13. Enzymes for Food and Energy. 13.1 Enzymes for Food Industry. 13.2 Enzymes for Bioenergy.

14. Enzymes for Biosensors. 14.1 Enzymatic Measurement. 14.2 Applications of Enzyme Biosensors.

15. Enzymes for Environment. 15.1 Enzymatic Removal of Pollutants. 15.2 Carbon Dioxide Conversion.