Main description:

This reference book comprehensively reviews the significance of DNA technology in forensic science. After presenting the theory, basic principles, tools and techniques that are used in forensic DNA typing, it summarizes various techniques, including autosomal STR, Y-STR, X-STR, mitochondrial DNA and NGS, used in solving both criminal as and civil cases, such as paternity disputes, identification of mutilated remains, and culprit identification in sexual assault and murder cases. It also provides an overview of DNA-based genetic diagnostics for various diseases, and discusses the role of DNA typing in drug reactions, as well as the application of non-human DNA profiling of animals and plants in forensic science investigations. Lastly, the book examines the role of internal quality control in maintaining the high quality of DNA profiling.

Contents:

1. Introduction to forensic DNA typing

2. Biological sources of DNA: the target materials for forensic DNA analysis

3. Collection, preservation and transportation of DNA evidences

4. Tools and techniques used in forensic DNA typing

5. Forensic DNA fingerprinting: evaluation of the currently used autosomal STR markers and kits

6. Forensic DNA fingerprinting: evaluation of the currently used gonosomal markers and kits

7. Usefulness of SNPs in forensic DNA analysis

8. DNA databases: A boon to forensic DNA analysis

9. Statistical evaluation of forensic DNA evidence

10. Potential of DNA technique based body fluid identification

11. DNA fingerprinting and legislation

12. A glimpse of famous cases in history solved by DNA typing

13. Usefulness of autosomal STR analysis in solving cases: a case study

14. A case study on the usefulness of Y-STR typing technique

15. Usefulness of X-STR analysis in solving cases: a case study

16. Usefulness of mitochondrial DNA analysis in solving cases: a case study

17. Application of NGS in solving mysterious cases

18. A case study on disputed paternity solved by DNA typing

19. Identification of mutilated bodies: a case study

20. A case study on murder mystery solved by DNA typing

21. Identification of sexual assault culprit by DNA typing: a case study

22. Disaster victim identification by DNA typing

23. Cases of robbery solved by DNA typing

24. Ancient DNA analysis: A case study

25. Miscellaneous cases solved by DNA typing

26. DNA analysis for prenatal diagnosis

27. Analysis of DNA for diagnosis of neonatal diseases

28. Diseases of skin, hair and nail and their analysis through DNA

29. A Paediatrician's perspective of DNA analysis

30. Diseases of metabolism and endocrine disorder by DNA analysis

31. DNA analysis in malignancies; diagnostic and prognostic value

32. The role of DNA analysis in drug reactions

33. Detection of various diseases through DNA typing: case studies

Section V: Modifications in routine methodologies to solve challenging cases

34. Modification of the manual DNA extraction protocols to obtain results in a case

35. A modified protocol of automated DNA extraction technique to obtain results

36. Modification of PCR conditions to obtain results in a case

37. Modification of various parameters of capillary electrophoresis to obtain results in a case

38. Required modifications of default software parameters to obtain results in a case

39. Application of various statistical tools to draw conclusion in a case

40. Cases pertaining to wild-life crimes solved by DNA typing

41. Usefulness of plant DNA typing in solving different cases

42. Microbial typing to solve various cases

43. Touch DNA analysis

44. RAPID DNA analysis

45. Interpretation of mixed profiles

46. Role of population data in strengthening forensic DNA analysis

47. Role of genealogy databases for identification of suspected perpetrators

48. Familial searching: an application of forensic DNA profiling

49. An insight on miscarriages of justice through DNA typing

50. Validation kits and instruments and its necessity

51. Quality control in forensic DNA analysis

52. DNA phenotyping: the technique of future

53. Recommendations on report writing of DNA profiling results

54. The role of scientific societies in generating best practices in forensic DNA profiling