Main description:

Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation. As a volume in the highly successful Methods in Molecular Biology (TM) series, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.

Contents:

1. General Review on In Vitro Hepatocyte Models and their Applications
Christiane Guguen-Guillouzo and Andre Guillouzo

2. Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation
Anne Weber, Thomas Touboul, Sylvie Mainot, and Dominique Mahieu-Caputo

3. Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue
Edward L. LeCluyse and Eliane Alexandre

4. Optimization of the Cryopreservation of Primary Hepatocytes
Nicola J. Hewitt

5. Cryopreservation of Human Hepatocytes for Clinical Use
Ragai R. Mitry, Sharon C. Lehec, and Robin D. Hughes

6. Hepatocyte Differentiation
Katy M. Olsavsky Goyak, Elizabeth M. Laurenzana, and Curtis J. Omiecinski

7. Reversible Manipulation of Apoptosis Sensitivity in Cultured Hepatocytes by Matrix Mediated Manipulation of Signaling Activities
Patricio Godoy, Markus Schug, Alexander Bauer, and Jan G. Hengstler

8. Markers and Signaling Factors for Stem Cell Differentiation to Hepatocytes: Lessons from Developmental Studies
Frederic Lemaigre

9. Hepatic Stem Cells
Robert E. Schwartz and Catherine Verfaillie

10. Hepatic Stem Cells and Liver Development
Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, and Naoya Kobayashi

11. Generation of Hepatocytes from Human Embryonic Stem Cells
Neta Lavon

12. Isolation and Culture of Adult Human Liver Progenitor Cells: In vitro Differentiation to Hepatocyte-Like Cells
Sabine Gerbal-Chaloin, Cedric Duret, Edith Raulet, Francis Navarro, Pierre Blanc, Jeanne Ramos, Patrick Maurel, and Martine Daujat-Chavanieu

13. The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies
Marie-Jeanne Marion, Olivier Hantz, and DavidDurantel

14. Transdifferentiation of Pancreatic Cells to Hepatocytes
Chia-Ning Shen and David Tosh

15. Evaluation of Drug Metabolism, Drug-Drug Interactions, and In vitro Hepatotoxicity with Cryopreserved Human Hepatocytes
Albert P. Li

16. The Use of Human Hepatocytes to Investigate Drug Metabolism and CYP Enzyme Induction
Sylvie Klieber, Francois Torreilles, Francois Guillou, and Gerard Fabre

17. The Use of Hepatocytes to Investigate UDP-Glucuronosyltransferases and Sulfotransferases
Sylvie Fournel-Gigleux, Michael W.H. Coughtrie, Mohamed Ouzzine, and Jacques Magdalou

18. The Use of Hepatocytes to Investigate Drug Uptake Transporters
Kazuya Maeda and Yuichi Sugiyama

19. Metabonomic Studies on Human Hepatocyte in Primary Culture
Vincent Croixmarie, Thierry Umbdenstock, Olivier Cloarec, Amelie Moreau, Jean-Marc Pascussi, Yannick Parmentier, Claire Boursier-Neyret, and Bernard Walther

20. The Application of HepRG Cells in Evaluation of Cytochrome P450 Induction Properties of Drug Compounds
Tommy B. Andersson

21. The Use of Hepatocytes to Investigate Drug Toxicity
Maria Jose Gomez-Lechon, Jose V. Castell, and Maria Teresa Donato

22. The Use of Human Hepatocytes to Investigate Bile Acid Synthesis
Ewa C. S. Ellis and Lisa-Mari Nilsson

23. The Use of Primary Human Hepatocytes to Investigate Blood Coagulation Factor Secretion and Activity
Christine Biron-Andreani, Edith Raulet, Lydiane Pichard-Garcia, and Patrick Maurel

24. The Use of Human Hepatocytes to Investigate HCV Infection
Lydiane Pichard-Garcia, Philippe Briolotti, Dominique Larrey, Antonio Sa-Cunha, Bertrand Suc, Sylvain Laporte, and Patrick Maurel

25. The Use of Hepatocytes to Investigate HDV Infection: The HDV/HepaRG Model
Camille