Main description:

Now in its fourth edition, Histological and Histochemical Methods has been expanded and updated with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained in simple, descriptive terms. This book is recommended for lecturers and students in biological and biomedical disciplines using histological and histochemical techniques. Postgraduate researchers will find this to be the ideal laboratory companion, as will medical laboratory technologists preparing for their professional examinations. Reviews: "John Kiernan has published extensively on histochemical methods since his first scientific paper in 1964. His textbook encompasses his wide personal experience and interest in dyes, staining and histochemical methodologies...I personally believe that this text should be found on the bookshelves of every histology and histochemistry laboratory as a basic reference source."
Raymond Coleman, Acta Histochemica, April 2010

Contents:

1. Introduction to microtechnique 2. Fixation 3. Decalcification and other treatments for hard tissues 4. Processing and mounting 5. Dyes 6. Histological staining in one or two colours 7. Staining blood and other cell suspensions 8. Methods for connective tissue 9. Methods for nucleic acids 10. Organic functional groups and protein histochemistry 11. Carbohydrate histochemistry 12. Lipids 13. Methods for inorganic ions 14. Enzyme histochemistry: general considerations 15. Hydrolytic enzymes 16. Oxidoreductases 17. Methods for soluble organic compounds of low molecular weight 18. Metal reduction and precipitation methods 19. Immunohistochemistry 20. Miscellaneous data Bibliography Glossary Index