Main description:

This second edition presents an up-to-date overview of the current state-of-the-art protocols and aims to miRNomics into a broader perspective. Chapters detail methods and techniques ranging from miRNA biogenesis, their biological function, computational analyses to their medical implications and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, miRNomics: MicroRNA Biology and Computational Analysis, Second Edition ensures successful results in the further study of this vital field.

Contents:

1 Regulation of MicroRNAs Kemal Ergin and Rahmi Cetinkaya

2 Experimental MicroRNA Detection Methods Bilge Yaylak and Bunyamin Akgul

3 Functional Annotation of MicroRNAs using Existing Resources Harsh Dweep, Louise C. Showe, and Andrew V. Kossenkov

4 Experimental MicroRNA Targeting Validation Bala Gur Dedeoglu and Senem Noyan

5 Endogenous miRNA Sponges Ayse Hale Alkan and Bunyamin Akgul

6 MicroRNA Targeting Hossein Ghanbarian, Mehmet Taha Yildiz, and Yusuf Tutar

7 MicroRNA Databases and Tools Tharcisio Soares de Amorim, Daniel Longhi Fernandes Pedro, and Alexandre Rossi Paschoal

8 Computational Detection of Pre-microRNAs Muserref Duygu Sacar Demirci

9 Computational Methods for Predicting Mature MicroRNAs Malik Yousef, Alisha Parveen, and Abhishek Kumar

10 Computational Detection of MicroRNA Targets Pedro Gabriel Nachtigall and Luiz Augusto Bovolenta

11 Evolution and Phylogeny of MicroRNAs --- Protocols, Pitfalls, and Problems Cristian A. Velandia-Huerto, Ali M. Yazbeck, Jana Schor, and Peter F. Stadler

12 Ensemble Classifiers for Multi-class MicroRNA Classification Luise Odenthal, Jens Allmer, and Malik Yousef

13 MicroRNAs in Genetic Etiology of Human Diseases Melis Olcum, Kemal Ugur Tufekci, and Sermin Genc

14 The role of Exosomal MicroRNAs in Cell-to-Cell Communication Bora Tastan, Emre Tarakcioglu, Yelda Birinci, Yongsoo Park, and Sermin Genc

15 MicroRNAs and Heat Shock Proteins in Breast Cancer Biology Mehmet Taha Yildiz, Lutfi Tutar, Nazli Irmak Giritlioglu, Banu Bayram and Yusuf Tutar

16 Role of MicroRNAs in Extreme Animal Survival Strategies Hanane Hadj-Moussa, Liam J. Hawkins, and Kenneth B. Storey

17 Computational and Bioinformatics Methods for MicroRNA Gene Prediction Ege Riza Karagur, Sakir Akgun, and Hakan Akca

18 The Role of MiRNA in Cancer: Pathogenesis, Diagnosis, and Treatment Erez Uzuner, Gizem Tugce Ulu, Sevim Beyza Gurler, and Yusuf Baran

19 Current Challenges in miRNomics Bunyamin Akgul, Peter F Stadler, Liam J Hawkins, Hanane Hadj-Moussa, Kenneth B Storey, Kemal Ergin, Rahmi Cetinkaya, Alexandre R Paschoal, Pedro G Nachtigall, Yusuf Tutar, Malik Yousef, and Allmer Jens