Main description:

This second edition volume is a companion volume to the previous edition and looks at new findings on novel bioactive chemicals and cognate targets, as well as the use of synthetic small molecules and a variety of tools to understand these processes. Chapters in this book cover topics such as screening plants for novel bioactive chemicals including lipid signaling and photo receptors; small molecule screens to include peptide ligands to generate new variations; using new chemicals to affect and dissect hormone signaling; and the application of easier microscale methods to simplify target identification and validation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Plant Chemical Genomics: Methods and Protocols, Second Edition is a valuable resource that provides a foundation of techniques for novice and expert researchers in the plant chemical biology community.

Contents:

Preface...
Table of Contents...
Contributing Authors...

Part I Screening for Novel Small Molecules

1. Chemical Genetics to Uncover Mechanisms Underlying Lipid-Mediated Signaling Events in Plants
Bibi Rafeiza Khan, Kent D. Chapman, and Elison B. Blancaflor

2. Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitor
Emiko Okubo-Kurihara, WenDee Ong, Yukio Kurihara, Natsumaro Kutsuna, and Minami Matsui

3. Whole Seedling Based Chemical Genetic Screens in Arabidopsis
Shuai Huang and Xin Li

4. Identification of Type iii Secretion Inhibitors for Plant Disease Management
Roger de Pedro Jove, Pau Sebastia, and Marc Valls

5. Investigation of Drug Efficacy by Screening Bioactive Chemical Effects on Plant Cell Subcellular Architecture
Di Sansebastiano Gian Pietro

Part II New Approaches to Variation: Peptide Screen for Novel Effectors

6. In Vivo Chemical Genomics with Random Cyclized Peptides
Tautvydas Shuipys, Maureen Clancy, Elizabeth Estrada-Johnson, and Kevin M. Folta

7. Interfering Peptides Targeting Protein-Protein Interactions in the Ethylene Plant Hormone Signaling Pathway as Tools to Delay Plant Senescence
Alexander Hofmann, Alexander Minges, and Georg Groth

Part III Chemical Genomics in Hormone Signaling

8. The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling
Kjell De Vriese, Long Nguyen, Simon Stael, Dominique Audenaert, Tom Beeckman, and Steffen Vanneste

9. Identification of ABA Receptor Agonists Using a Multiplexed High-Throughput Chemical Screening
Jorge Lozano-Juste, Irene Garcia-Maquilon, Jose Brea, Rocio Pina, Armando Albert, Pedro L. Rodriguez, and Maria Isabel Loza

10. A Luciferase Reporter Assay to Identify Chemical Activators of ABA Signaling
Irene Garcia-Maquilon, Pedro L. Rodriguez, Aditya S. Vaidya, and Jorge Lozano-Juste

11. Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening
Chanung Park, Dong Hye Seo, Joseph Buckley, and Gyeong Mee Yoon

12. Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors
Kazuo Soeno, Akiko Sato, and Yukihisa Shimada

Part IV Target Identification and Confirmation Approaches

13. Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach
Dan-Dan Liu, Chang Zou, Jianbin Zhang, Peng Gao, Yongping Zhu, Yuqing Meng, Nan Ma, Ming Lyu, Chengchao Xu, Qingsong Lin, and Jigang Wang

14. Label-Free Target Identification and Confirmation Using Thermal Stability Shift Assays
Cecilia Rodriguez-Furlan and Glenn R. Hicks

15. Drug Affinity Responsive Target Stability (DARTS) Assay to Detect Interaction Between a Purified Protein and a Small Molecule
Lei Huang, Diwen Wang, and Chunhua Zhang

16. Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein
Xiaohui Li and Chunhua Zhang

17. Microscale Thermophoresis (MST) to Detect the Interaction between Purified Protein and Small Molecule
Lei Huang and Chunhua Zhang

Subject Index List...