Main description:

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. RNA and CRISPR Interferences: Past, Present and Future Perspectives
Mouldy Sioud

2. Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents
Kim A. Lennox and Mark A. Behlke

3. Preparation, Determination of Activity, and Biodistribution of Cholesterol-Containing Nuclease-Resistant siRNAs In Vivo
Ivan V. Chernikov, Mariya I. Meschaninova, and Elena L. Chernolovskaya

4. Multifunctional Nanodelivery Platform for Maximizing Nucleic Acids Combination Therapy
Seung Koo Lee, Benedict Law, and Ching-Hsuan Tung

5. PAMAM Dendrimers as a Delivery System for Small Interfering RNA
Shiva Kheiriabad, Maryam Ghaffari, Jafar Ezzati Nazhad Dolatabadi, and Michael R. Hamblin

6. Delivery of Functional Small RNAs via Extracellular Vesicles In Vitro and In Vivo
Duo Zhang, Heedoo Lee, and Yang Jin

7. Optimized siRNA Delivery into Primary Immune Cells using Electroporation
Mouldy Sioud

8. Synthesis and Evaluation of Caged siRNAs with Single cRGD Modification for Photoregulating RNA Interference
Lijia Yu, Duanwei Liang, Nannan Jing, Changmai Chen, and Xinjing Tang

9. Exploring 5'-Biotinylation of the Sense Strand to Improve siRNA Specificity and Potency
Anne Mobergslien and Mouldy Sioud

10. In Vivo Delivery of Cassettes Encoding Anti-HBV Primary MicroRNAs using an Ancestral Adeno-Associated Viral Vector
Njabulo Mnyandu, Patrick Arbuthnot, and Mohube Betty Maepa

11. Generating DNA Expression Cassettes Encoding Multimeric Artificial MicroRNA Precursors
Fiona T. van den Berg, Abdullah Ely, and Patrick Arbuthnot

12. MiR-302-Mediated Somatic Cell Reprogramming and Method for Generating Tumor-Free iPS Cells Using miR-302
Shi-Lung Lin, Jack S. Chen, and Shao-Yao Ying

13. Urinary MicroRNAs as Emerging Class of Noninvasive Biomarkers
Jaroslav Juracek and Ondrej Slaby

14. Improving Dendritic Cell Cancer Vaccine Potency using RNA Interference
Stein Saeboe-Larssen and Mouldy Sioud

15. Unleashing the Therapeutic Potential of Dendritic and T Cell Therapies using RNA Interference
Mouldy Sioud

16. Harnessing the Antiviral-Type Responses Induced by Immunostimulatory siRNAs for Cancer Immunotherapy
Per Ole Iversen and Mouldy Sioud

17. Cancer Immunotherapy: Targeting Tumor-Associated Macrophages by Gene Silencing
Karin Zins and Dietmar Abraham

18. Use of RNA Interference with TCR Transfer Enhance Safety and Efficiency
Nicholas Paul Casey, Jon Amund Kyte, and Hiroshi Fujiwara

19. CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity
Kasidet Hiranniramol, Yuhao Chen, and Xiaowei Wang

20. CRISPR/Cas9 Genome Editing in Human Cell Lines with DONOR Vector Made by Gibson Assembly
Nirakar Sahoo, Victoria Cuello, Shreya Udawant, Carl Litif, Julie A. Mustard, and Megan Keniry

21. Genome Editing in Zebrafish using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task
Sergey V. Prykhozhij, Anna Cordeiro-Santanach, Lucia Caceres, and Jason N. Berman

22. Next Generation of Adoptive T Cell Therapy using CRISPR/Cas9 Technology: Universal or Boosted?
Sebastien Walchli and Mouldy Sioud

23. Engineering T-Cells using CRISPR/Cas9 for Cancer Therapy
Xingying Zhang, Chen Chen, Wen Sun, and Haoyi Wang

24. CRISPR/Cas9 Mediated Genome Engineering of Primary Human B Cells
Kanut Laoharawee, Matthew J. Johnson, and Branden S Moriarity

25. Gene Editing in B-Lymphoma Cell Lines using CRISPR/Cas9 Technology
Baoyan Bai, June Helen Myklebust, and Sebastien Walchli

26. Gene Knockout in Hematopoietic Stem and Progenitor Cells followed by Granulocytic Differentiation
Perihan Mir, Malte Ritter, Karl Welte, Julia Skokowa, and Maksim Klimiankou

27. CRISPR/Cas9 Genome Editing of Human Induced Pluripotent Stem Cells followed by Granulocytic Differentiation
Benjamin Dannenmann, Masoud Nasri, Karl Welte, and Julia Skokowa