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Main description:
As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of "Natural Killer Cell Protocols: Cellular and Molecular Methods" brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, "Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition" seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells.
Contents:
1. Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils
Aharon G. Freud and Michael A. Caligiuri
2. In Vitro Development of Human Killer-Immunoglobulin Receptor Positive NK Cells
Frank Cichocki and Jeffrey S. Miller
3. Subset Analysis of Human and Mouse Mature NK Cells
Yoshihiro Hayakawa, Daniel M. Andrews, and Mark J. Smyth
4. Assessing Licensing of NK Cells
A. Helena Jonsson and Wayne M. Yokoyama
5. Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development in vivo
Rebecca H. Lian and Vinay Kumar
6. Use of Transfected Drosophila S2 Cells to Study NK Cell Activation
Michael E. March, Catharina C. Gross, and Eric O. Long
7. Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry
Deborah N. Burshtyn and Chelsea Davidson
8. Studying NK Cell / Dendritic Cell Interactions
Mathias Lucas, Cedric Vonarbourg, Peter Aichele, and Andreas Diefenbach
9. Analysis of the NK Cell Immunological Synapse
Keri B. Sanborn, Gregory Rak, Ashley Mentlik, Pinaki P. Banerjee, and Jordan S. Orange
10. Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry
Alexander W. MacFarlane IV, James F. Oesterling, and Kerry S. Campbell
11. Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells
Takuya Miyagi, Seung-Hwan Lee, and Christine A. Biron
12. A Model System for Studying NK Cell Receptor Signaling
Lukasz K. Chlewicki and Vinay Kumar
13. Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction
S. M. Shahjahan Miah and Kerry S. Campbell
14. Lentiviral Gene Transduction inHuman and Mouse NK Cell Lines
Ram Savan, Tim Chan, and Howard A. Young
15. Introduction of shRNAs into Human NK-like Cell Lines with Retrovirus
Amanda K. Purdy and Kerry S. Campbell
16. Introduction of shRNAs into Primary NK Cells with Lentivirus
Sam K.P. Kung
17. Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands
Dikla Lankry, Roi Gazit, and Ofer Mandelboim
18. Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation
Alon Zilka, Michal Mendelson, Benyamin Rosental, Oren Hershkovitz, and Angel Porgador
19. Identification of NK Cell Receptor Ligands Using a Signaling Reporter System
Yoshie-Matsubayashi Iizuka, Nikunj V. Somia, and Koho Iizuka
20. Determining Ligand Specificity of Ly49 Receptors
Kerry J. Lavender and Kevin P. Kane
21. Probing the Interactions of NK Cell Receptors with Ligand Expressed in trans and cis.
Jonathan Back, Leonardo Scarpellino, and Werner Held
22. A Simple Method to Measure NK Cell Cytotoxicity in vivo
Aurore Saudemont, Shannon Burke, and Francesco Colucci
23. Functional Analysis of Human NK Cells by Flow Cytometry
Yenan T. Bryceson, Cyril Fauriat, Joao M. Nunes, Stephanie M. Wood, Niklas K. Bjoerkstroem, Eric O. Long, and Hans-Gustaf Ljunggren
24. Analysis of the KIR Repertoire in Human NK Cells by Flow Cytometry
Niklas K. Bjoerkstroem, Cyril Fauriat, Yenan T. Bryceson, Johan K. Sandberg, Hans-Gustaf Ljunggren, and Karl-Johan Malmberg
25. KIR Genotyping by Multiplex PCR-SSP
Smita Kulkarni, Maureen P. Martin, and Mary Carrington
26. Identification and Analysis of Novel Transcripts and Promoters in the Human Killer Cell Immunoglobulin-like Receptor (KIR) Genes
Hongchuan Li, Paul W.
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2009
Pages: 564
Weight: 1373g
Availability: Available
Subcategories: Immunology
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