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Main description:
The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Contents:
General Strategies
Shawn Doonan and Paul Cutler
Preparation of Extracts From Animal Tissues
J. Mark Skehel
Protein Extraction From Plant Tissues
Roger J. Fido, E. N. Clare Mills, Neil M. Rigby, and Peter R. Shewry
Extraction of Recombinant Protein From Bacteria
Anne F. McGettrick and D. Margaret Worrall
Protein Extraction From Fungi
Paul D. Bridge, Tetsuo Kokubun, and Monique S. J. Simmonds
Subcellular Fractionation of Animal Tissues
Norma M. Ryan
Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria
Alyson K. Tobin and Caroline G. Bowsher
The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds
William S. Pierpoint
Avoidance of Proteolysis in Extracts
Robert J. Beynon and Simon Oliver (Revised by Paul Cutler)
Concentration of Extracts
Shawn Doonan
Making and Changing Buffers
Shawn Doonan
Purification and Concentration by Ultrafiltration
Paul Schratter (Revised by Paul Cutler)
Bulk Purification by Fractional Precipitation
Shawn Doonan
Ion-Exchange Chromatography
Chris Selkirk
Hydrophobic Interaction Chromatography
Paul A. O'Farrell
Affinity Chromatography
Paul Cutler
Dye-Ligand Affinity Chromatography
Anne F. McGettrick and D. Margaret Worrall
Lectin Affinity Chromatography
Iris West and Owen Goldring (Revised by Paul Cutler)
Immunoaffinity Chromatography
Paul Cutler
Immobilized Metal-Ion Affinity Chromatography
Tai-Tung Yip and T. William Hutchens (Revised by Paul Cutler)
Chromatography on Hydroxyapatite
Shawn Doonan
Thiophilic Affinity Chromatography and Related Methods
Paul Matejtschuk
Affinity Precipitation Methods
Jane A. Irwin and Keith F. Tipton
Isoelectric Focusing
Reiner Westermeier
Chromatofocusing
Timothy J. Mantle and Patricia Noone
Size-Exclusion Chromatography
Paul Cutler
Fast Protein Liquid Chromatography
David Sheehan and Siobhan O'Sullivan
Reversed-Phase Chromatography of Proteins
William A. Neville
Extraction of Membrane Proteins
Kay Ohlendieck
Removal of Detergent From Protein Fractions
Kay Ohlendieck
Purification of Membrane Proteins
Kay Ohlendieck
Lyophilization of Proteins
Ciaran O'Fagain
Storage of Pure Proteins
Ciaran O'Fagain
Electroelution of Proteins From Polyacrylamide Gels
Michael J. Dunn
Electroblotting of Proteins From Polyacrylamide Gels
Michael J. Dunn
Two-Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses
Neil A. Jones
Microscale Solution Isoelectrofocusing: A Sample Prefractionation Method for Comprehensive Proteome Analysis
Xun Zuo and David W. Speicher
Practical Column Chromatography
Shawn Doonan
Detection Methods
Jacek Mozdzanowski and Sudhir Burman
Peptide Proteomics
Dean E. McNulty and J. Randall Slemmon
Multidimensional Liquid Chromatography of Proteins
Rod Watson and Tim Nadler
Mass Spectrometry
David J. Bell
Purification of Therapeutic Proteins
Julian Bonnerjea
Purification Process Scale-Up
Karl Prince and Martin Smith
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 496
Weight: 918g
Availability: Available
Subcategories: Biochemistry
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